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| Synonym: | Deoxyribonucleate 5'-oligonucleotido-hydrolase, DNAse I |
| Enzyme Commission (EC) Number: | 3.1.21.1 ( BRENDA | IUBMB ) |
Description
| Application | Used for the removal of DNA from protein samples. |
| Biochem/physiol Actions | Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5' phosphates and 3' OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved. |
| Features and Benefits | • RNA purification by removing DNA
• Prepare DNA for nick translation1
• Footprinting assays to determine DNA-protein interactions2
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| Preparation Note | This product is prepared essentially free of RNase and protease activity. |
| Physical form | Supplied as a solution in 20 mM HEPES, 10 mM CaCl2, 10 mM MgCl2, 1 mM DTT, 50% glycerol, pH 7.5. |
Properties
| biological source | from bovine |
| recombinant | expressed in proprietary host |
| mol wt | mol wt ~39 kDa |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
References
| reference | Rigby, P.W., et al. J. Mol. Biol. 113, 237, (1977) |
| | Galas, D. J. and Schmitz, A., DNase footprinting: a simple method for the detection of protein-DNA binding Nucleic Acids Res. 5, 3157, (1978) |
